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Proteins were then separated via SDS-PAGE and transferred to PVDF membranes, which were blocked with 5% skim milk for 1 h at 25 C before incubation with primary antibodies for 12 h at 4 C

Real time-PCR (RT-PCR) results revealed that peroxynitrite treatment caused significant increases in the mRNA expression of proapoptotic genes Bax and P53 , and decreases in the mRNA expression of antiapoptotic genes Bcl2 and Bcl-xL compared with controls (Figure 8D)

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Machine learning (ML) models are particularly powerful in predicting nanomaterialcell interactions, thereby accelerating the development of personalized therapies [13]