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All restriction enzymes used in the study were purchased from New England Biolabs

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Nrf2 Nuclear factor-erythroid-2-related factor 2 the Withania somnifera active ingredient has at least 25 fold, at least 30 fold, at least 35 fold, at least 40 fold, at least 45 fold, at least 50 fold, at least 55 fold, at least 60 fold, at least 65 fold, at least 70 fold, at least 75 fold, at least 80 fold, at least 85 fold, at least 90 fold, at least 95 fold, at least 100 fold, at least 110 fold, at least 120 fold, at least 130 fold, at least 140 fold at least 150 fold, or at least 200 fold Nuclear factor-erythroid-2-related factor 2 (Nrf2) transcription factor-activating potential as compared to an untreated control