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The cells were washed once in with 50 L PBS (Gibco, #14190-094) after which 30 L of each concentration from the dilution series was added to the 384-well assay plate with cells

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Pituitary primordia were explanted from 11-day-old rat fetuses and cultured in a synthetic medium enriched with either DSIP at several concentrations, GnRH (10(-9) M) or TRH (10(-9) M)

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