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It must be avoided by people with hypertension, cardiovascular disease, hyperthyroidism, diabetes, glaucoma, or those taking MAOIs, and a 2024 MHRA/EMA safety update warns of rare but serious neurological risks including PRES and RCVS

Over 100 placebo-controlled trials suggest that use of NRT doubles a smoker's chances of quitting successfully to 612 months (see Silagy et al., 2005, for a meta-analysis)

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Multigene Agrobacterium-Mediated Tobacco Transformation for Drought Tolerance Recent studies demonstrate that Agrobacterium-mediated leaf-disk transformation efficiently delivers multigene constructs into Nicotiana tabacum for abiotic-stress engineering