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D-Nle-Tyr-Ile-NH-(CH 2 ) 5 -CONH 2 exhibited no detectable metabolism over 90 min in an in-vitro metabolism assay using rat liver microsomes (data not shown)
Subsequently, fertilized oocytes from each group were transferred individually to embryo culture droplets as SOF medium supplemented with 0.5 mM sodium citrate, 1 mM sodium pyruvate, 1% non-essential amino acids, 2% essential amino acids, 2 mM Gluta MAX , 2.8 mM myo-inositol and 4 mg/ml bovine serum albumin covered by mineral oil in a 35 mm sterile dish, followed by embryo culture in a CO 2 incubator (38.5C, 5%O 2 and 5%CO 2 ) ( After 48 h, cleaved two-cell embryos from each group were microscopically observed with cleavage rates calculated as the percentage of cleaved two-cell embryos/MII oocytes
42 MaggsJ
2016 Apr;41(2):214-9. PMID: 26931055
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