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Considering ITB represents a key element in interpreting these studies since patients with precipitated death or with an earlier disease progresses rarely develop irAEs

It is evident from the isoelectric focusing process that regardless of where the sample is applied on the gel, proteins of various kinds can migrate towards their respective isoelectric points and ultimately halt there, without affecting the final electrophoretic results

Any contamination can quickly degrade the peptide, compromise its molecular structure, and ultimately make your research findings completely unreliable

Evidence-based comparison guide