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This result was corroborated by the analysis of ALT and AST liver enzymes, which overflow to the extracellular medium due to membrane permeability alterations after cellular injury

Transmission electron microscopy and BODIPY staining Samples collected from each infection stage were fixed in 2.5% ( v/v ) glutaraldehyde at 4 C overnight, and were washed with 0.1 M phosphate buffer saline (PBS), post-fixed in 1% ( w/v ) osmic acid for 2 h, washed again with 0.1 M PBS, dehydrated sequentially with ethanol gradient, and infiltrated with epoxy

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