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Cells were harvested using 110 L ice cold lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche)

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In preclinical models, NNMT inhibition has been shown to mimic and boost exercise-mediated improvements in muscle function, even in sedentary subjects

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Place the cap on the needle and remove

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