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10.3389/fcvm.2025.1511747 153 XinM.XuA.TianJ.WangL.HeY.JiangH.et al (2024)

Traditional methods such as liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS), which integrate chromatography and mass spectrometry, are currently the mainstream techniques for detecting metabolites in tumors, but they face limitations in terms of low detection efficiency for metabolites with strong polarity, isomerism, and low ionization efficiency