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Blots were rinsed thrice (0.1% Tween20 in Tris-buffered saline, 5 min each), followed by incubation with peroxidase-conjugated secondary antibody (Dako) for 2 h at room temperature

Rat studies using collagen tubes containing GHK-Cu showed increased nerve fiber regeneration, increased axon count, increased Schwann cell proliferation, and restored skin innervation in healing wounds