We used CSOP to detect the effect of solution crowding on height by measuring a long multi-FN3-domain protein (9L) under varying amounts of 8kDa polyethylene glycol (PEG) in solution
The bright yellow to fluorescent green color sometimes seen when taking nutritional supplements is primarily caused by some excess nutrients being excreted (particularly riboflavin and other B vitamins), and it may continue to vary in color based on your hydration and nutritional need
Hydroquinone is a prescription-strength bleaching agent that inhibits melanin production but carries risks including skin irritation, paradoxical darkening with prolonged use, and ochronosis with very long-term use
METHOD DETAILS Antibodies and reagents Antibodies against the following proteins were used (See also Key Resource Table): Cell Signaling: FOXO1 (2880), FOXO4 (9472), Phospho-Ser15 p53 (9286), PUMA (4976), BIM (C3C5), Cleaved Caspase-3 (9661), Sigma: FOXO4 (HPA040232), Tubulin (clone B512