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The PVDF membranes were incubated with primary antibodies overnight at 4 C, incubated with secondary antibodies at room temperature for 1 h, and visualized using the Bio-Rad ChemiDoc XRS + system

Special Population Considerations Certain populations may need modified protocols

For the well plate-based drug tests, all compounds were diluted in basal culture media (DMEM, TSM-basal medium, or EAPM accordingly) to reach final compound concentrations of 0.5 nM, 1 nM, 10 nM, 100 nM, 500 nM, 1 M, and 10 M

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