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Next, specimens were dehydrated through an ascending ethanol series, starting with 50% ethanol, followed by incubation in 70% ethanol, 1% uranyl acetate (#21447, Polyscience Europe, Heidelberg, Germany), and 1% phosphotungstic acid (#455970, Merck, Darmstadt, Germany) solution overnight at 4 C

Demographic trends: Use is slightly higher among men aged 2544, particularly those working in trades or rural industries where traditional tobacco use remains part of workplace culture

In evidence, Mr Fagan accepted that he broke the law and Aer Linguss procedures

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