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The cumulative release calculation method involved determining the percentage of berberine released over time from the nanoformulation, using the following formula [47]: Where Pt = percentage release at time t and P (t 1) = percentage release previous to t. In vivo pharmacokinetic study Berberine-loaded chitosan/SLN nanoparticles and free berberine were gavaged (50 mg/kg) in two groups (n = 10 rats per group) of female Wistar-Albino rats

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TABLE 1 Primer sequences

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