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The MMKD also emphasizes low-fat protein sources, such as fish and lean meats, as well as healthy fats such as extra virgin olive oil

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Cell and tissue cultures Cells were cultured in Eagle's minimum essential medium supplemented with 10% (v/v) fetal bovine serum, 1% non-essential amino acids, 2 mM l-glutamine, 100 U/mL penicillin and 100 mg/mL streptomycin at 37 C in a humidified atmosphere of 5% CO 2

The beyond use date for an opened or entered (e.g., needle punctured) multiple-dose container with antimicrobial preservatives is 28 days, unless otherwise specified by the manufacturer