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c) Diacylglycerol (DAG) secretion which activates protein Kinase-C

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doi: 10.3168/jds.2012-5790 94 OsorioJ

This works when the dose range is narrow and lands on convenient unit marks, but community sources note that measurement errors at high concentrations have proportionally larger consequences

Cell pellets were later thawed and lysed with RLT Plus lysis buffer, and total RNA was extracted with the Qiagen RNeasy kit according to the manufacturers instructions (Qiagen, 74134)