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Briefly, 400 ]x slices were cultured on (Miiipore, Billerica, MA) for 3 days after which they were biolistically transfected with tomato fluorescent protein (TFP) using a Helios Gen Gun (BioRad, Hercules, CA), according to the manufacturer's protocol, to visualize dendritic arbors

All Cenexa peptides are manufactured 100% end-to-end here in the USA using the Lyophilization (Freeze Drying) process

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In the D-Gal + FOXO4-DRI group, mice also received D-galactose injections and, after 4 weeks, were given intraperitoneal injections of FOXO4-DRI (5 mg/kg, MCE, HY-P4157) every 2 days for 4 weeks, followed by tissue collection