( A and B ) Immunoblot of antiPPAR coactivator 1- (anti-PGC1) following immunoprecipitation with anti-Ac-lysine antibody of acetylated-PGC1 (Ac-PGC1) and of the heavy IgG chain ( A ) and sirtuin 1 (SIRT1) and -CTIN protein levels ( B ) in hearts obtained from C57BL/6 mice fed on regular or tesaglitazar-containing chow (0.5 mol/kg bw) diet for 6 weeks (densitometric analysis is shown in Supplemental Figure 5, A and B
The whole process is fast and efficient Very effective
Suppliers operating online typically market AOD-9604 as a "research chemical" or "laboratory use only" product to circumvent regulations, though end-use marketing to consumers for weight loss is widespread
The experimental data is listed in table 11.Table 11 The example data of preparation of L-carnitine from L-3-cyano-2-hydroxypropyl trimethylammonium having different optical purity Example 1 Establish the detecting method of the optical purity of epichlorohydrin with gas chromatography Example 2 Detection of the content of the D-isomer of the intermediate (L-3-cyano -2 - hydroxypropyl trimethyl ammonium) Example 3 Preparation of L-carnitine started from S-epichlorohydrin having different optical purity Example 4 Preparation of L-carnitine from L-3-chloro-2-hydroxypropyl trimethylammonium Example 5 Preparation of L-carnitine from L-3-cyano-2-hydroxypropyl trimethylammonium Claims (8) A Preparation Method of high-Purity L-carnitine, characterized in that to prepare L-carnitine with a purity being above 97%, wherein the content of D-carnitine is below 2%, by detecting and controlling the content of chiral material and chiral intermediate during the whole synthetic process which is started from S-epichlorohydrin, wherein the method include the following steps:(1)Detect the content of optical isomers of S-epichlorohydrin with GC and chiral column, and control the content of laevoisomer of S-epichlorohydrin within the range of 0% -12% W/W;(2)Detect the specific rotation of the intermediate L-3-chloro-2-hydroxypropyl trimethylamine during the synthetic process with a polarimeter, and control the value within the range of -26.0 -29.4;(3)Detect the optical purity of L-3-cyan-2-hydroxypropyl trimethylamine of the intermediate mixture and the content of its dextroisomer with a chiral derivatization reagent, and control the content of the dextroisomer within the range of 03.6%W/W;wherein the said chiral derivatization reagent is the optically pure D- or L-compound of formula (II):wherein, the carbon atom marked with an asterisk is the chiral carbon atom
