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Transepithelial resistance measurements Caco-2 cells were seeded onto 12 mm polycarbonate membrane inserts (PCF membrane inserts, Millipore, Germany) and bathed in DMEM/F12 medium which contained phenol red, 10% FBS, penicillin (100 units/ml) and streptomycin (10 g/ml)

7, 8, 9, 10 As an example, mast cells are involved in autoimmune disorders and many of the proinflammatory and tissue destructive mediators produced by these cells have been directly implicated in the pathogenesis of rheumatoid arthritis and other rheumatic diseases