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Proximity ligation assay (PLA) The Duolink In Situ Red Starter Kit Mouse/Rabbit was used for PLA, in accordance with the manufacturers instructions

All steps were the same as for the 96-well plate assay except that the culture volume was 1 mL, the volumes of the fixative, staining solution, and acetic acid were scaled proportionally (0.5 mL fixative, 1.25 mL of crystal violet solution, and 1.25 mL of glacial acetic acid per tube), and the washes were performed by gentle pipetting (2 mL water per tube per wash) instead of by submersion

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Studies have shown decreases in oxidative stress markers and evidence of reduced cellular damage