aureus strains LAC, HG003, MW2, JE2, JE2-lux (JE2 lux ABDCE) (kindly provided by Roger Plaut, Center for Biologics Evaluation and Research, Silver Spring) 28 , G4 (kindly provided by Anthony Richardson, University of Pittsburgh, Pittsburgh) 35 and bacteraemia isolates 8 were routinely cultured in tryptic soy broth (TSB, Fisher Scientific) or MuellerHinton broth (MHB, Oxoid) at 37 C with shaking (225 r.p.m.) unless otherwise stated
Furthermore, because some NAPQI is created regardless of the function of the other 2 pathways, failure in the glutathione-dependent pathway is expected to result in the accumulation of NAPQI and subsequent toxicity, even if the other 2 pathways are functional.It is not surprising that both sulfation- and glutathione-dependent pathways are aberrant in the same population because they are metabolically connected [41,72,73]
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