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Insights from honeybee (Apis mellifera) and fly ( Drosophila melanogaster) nicotinic acetylcholine receptors: from genes to behavioral functions

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15 To further verify whether the increase in cholesterol levels induced by cigarette smoke in airway epithelial cells contributes to cigarette smoke-induced airway inflammation through ROS promotion, we treated HBE cells with water-soluble cholesterol and detected ROS levels in airway epithelial cells using flow cytometry (Figure 6A) and the secretion levels of inflammatory factors IL6 and IL8 using qPCR (Figure 6B)