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Similar to ROS, low to moderate levels of RNS play physiological roles in vasodilation, neurotransmission, and immune regulation, while their overproduction leads to pathological modifications of proteins, DNA, and lipids [65]

This approach aligns with the growing recognition that the future of pain management lies not in blocking pain signals indefinitely, but in actually healing the underlying pathology

The lysate was centrifuged at 13000 g at 4 C, and supernatant subjected to deproteinization procedure via TCA precipitation (Abcam