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Description

Thus, even by prolonged fasting, the glucose levels that cancer cells are exposed to are not expected to fall below ~12 mmol/l, a range of values that has been verified by using glucose-sensing bacteria engineered to express GFP, embedded at different depths within solid tumors 56

Tissues were then incubated in 10, 20, and 30% sucrose in 1 PBS, embedded in OCT compound (Tissue-Tek, Sakura Finetek, 4583), snap frozen on dry ice, and then stored frozen at 80C