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Shan B, Guo C, Zhou H, Chen J

identified three subtypes in liver cancer cells by integrating single-cell sequencing data and spatial transcriptomics data, namely, ARG1+ metabolism subtype (Metab-subtype), TOP2A+ proliferation phenotype (Prol-phenotype), and S100A6+ pro-metastatic subtype (EMT-subtype) (92)

Two-sided t-test was used to determine statistical significance between primary tumors and metastases ( Nr1h3 , *P = 0.0220 , Gstm1, *P = 0.0496 , Itih4, *P = 0.050)