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Its antagonism which can be overcome by increasing the concentration of acetylcholine at receptor sites of the effector organ (e.g., by using anticholinesterase agents which inhibit the enzymatic destruction of acetylcholine) The receptors antagonized by atropine are the peripheral structures that are stimulated or inhibited by muscarine (i.e., exocrine glands, smooth and cardiac muscle)

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Additionally, the expression of genes related to the immunosuppressive functions of Treg cells (such as IL-10, CTLA-4, and TGF-) was downregulated, which weakened their ability to inhibit CD8 + T cell activity