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Para el resto del artculo, los trminos glutatin y GSH se utilizarn de manera indiferenciada

TNF- secretion TNF- levels in the media of treated cells were measured by an enzyme-linked immunosorbent assay (ELISA) according to the manufacturers instructions (R&D Systems, Minneapolis, MN)

and the protein content comprises 2.9% (and 2.3% in canned or frozen spinach due to denaturation and solubilization during the blanching process), with good levels of essential amino acids (except for sulfurous amino acids such as methionine and tryptophan)

Data are expressed as mean standard deviation (SD) from at least three independent experiments unless otherwise specified in the figure legend