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Jost, C., Nitsche, C., Scholz, T., Roux, L

Cell culture HeLa cells were grown in DMEM supplemented with fetal bovine serum (FBS, 10%) and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 ) at 37 C in a 5% CO 2 environment

Incubation of recombinant HPV-16 E7 with cell lysate and SDS-PAGE analysis HaCaT cells were lysed in 50 mM Tris-Cl, pH 7.4 with 5 mM EDTA, 250 mM NaCl, and 0.1% Triton X-100 (lysis buffer) plus protease inhibitors (1 mM PMSF, 10 g/ml aprotinin and leupeptin) for 3040 min on ice

La dosis de L-Arginina (Sura Vitasan) para adultos es de 1 a 3 cpsulas vegetales al da media hora antes de las comidas, con agua o zumo, o segn indicacin del especialista