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After repeated washing the bound fraction was eluted with 250 mM imidazole (Fischer) in lysis buffer containing 10% glycerol (Acros)

This is partly due to allicin, a substance that forms when fresh garlic is crushed or damaged ( When given to mice in high amounts, allicin seems to fight Candida yeasts at a slightly less effective level than the antifungal drug fluconazole ( Test-tube research also indicates that garlic extract may reduce yeasts ability to attach to the cells lining your mouth ( However, garlic provides only tiny amounts of allicin, whereas most studies use high doses

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C domains are most commonly encoded in the same coding DNA sequence (CDS) as their acceptor molecules, upstream of the corresponding A domain and there are only very few examples were a C domain is located in the C terminal proximitiy of the preceding CDS in NRPS 32,33,34