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Next, 1 ml of overnight culture was added to 100 ml of fresh M9 medium containing 50 g ml 1 kanamycin, which was further cultured at 37 C, 160 rpm until OD 600 = 0.5

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Similar content being viewed by others Introduction Selenium is an essential trace element critical for various physiological processes, including immunomodulation, antioxidant defense 1 regulation of endocrine and metabolism 2

In addition, investigators could titrate insulin glargine based on their discretion between study visits