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They used a tool to apply pressure to the appendages and measured the point at which the murine models pulled their appendages away

10.21037/jgo-22-222 328 ZhaoR.XueM.LinH.SmithM.LiangH.WeilerH.et al (2024)

It may be possible to extend the rapidly occurring beneficial cytoprotective effect of these agents and to use them to prevent ischemic colitis lesions, in which specific activation of the collateral circulation can circumvent obstructions and reestablish the continuity of blood flow

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