The compound is also mentioned in his Flagrant podcast appearance (June 2022, around 2:16:00)
This same enzyme was subsequently identified by two other laboratories from database searches of proteins containing the conserved G X S X G pentapeptide motif and alpha/beta-hydrolase fold, and was named adipose triglyceride lipase (ATGL) (145) or iPLA2 (53, 145)
Pregnancy and lactation safety has not been systematically studied, and as with most cosmetic and research compounds lacking definitive pregnancy safety data, conservative practice typically suggests avoiding use during pregnancy unless clearly needed, though the natural occurrence of GHK-Cu in the body and minimal systemic absorption from topical use suggest risk is likely to be low
The mitochondrial matrix contains enzymes, proteins (including mtDNA, RNA), ribosomes, and metabolites critical for the TCA cycle and fatty acid oxidation (Kaasik et al., 2007