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Protein Expression in Acute Myelogenous Leukemia CD34+CD38- Stem Cells is Markedly Different from CD34+ and Bulk Leukemic Cells

Markers frequently observed include: Neutrophil extracellular traps (NET) (also seen in Babesiosis) Increased D-dimer in subsets Increased fibrin production The thin layer lining blood vessels, the endothelium, appears to remain inflamed long after acute infection resolves

Morris CR, Suh JH, Hagar W, Larkin S, Bland DA, Steinberg MH, Vichinsky EP, Shigenaga M, Ames B, Kuypers FA, Klings ES (2008) Erythrocyte glutamine depletion, altered redox environment, and pulmonary hypertension in sickle cell disease

Incubate cells at 4 C for 10 min followed by centrifugation for 10 min at 14,000 rpm and 4 C