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250 ng of DNase treated RNA (Sigma, AMPD1) was used for rRNA depletion using RiboCop rRNA Depletion Kit V1.2 (Lexogen, #144)

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Osredkar J

The Ang IV analogue peptide 4 and the Nle-Ang IV analogue 5 provide examples comprising two basic amino groups accessible for protonation in the N-terminal of the peptides and flanking hydrophobic 2-isopropyl and n-butyl side chains, respectively (Figure 2) (Sardinia et al., 1994)