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Nat Struct Mol Biol (2014) 21(5):4438.10.1038/nsmb.2807 72 McNallyREckMJ

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2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added