Advances in prenatal ultrasound have made it possible to identify fetuses with cleft lip as early as weeks 13 to 14 of pregnancy
Then, the total number of cells (DAPI or NucBlue Live positive, blue), the number of apoptotic cells (CellEvent Caspase-3/7 positive, green) and dead cells (PI positive, red, or both CellEvent Caspase-3/7 and PI positive, yellow, or NucGreen positive, green) were counted
In addition, the levels of Salicylic acid (SA) and Jasmonic acid (JA) increased significantly, the expression levels of the genes encoding transcription factor TGA (PG2023696), pathogenesis-related protein 1 (PR1) (PG0005111), and the transcription inhibitor Aux/IAA (PG0006093) all increased while the expression levels of jasmonate ZIM domain-containing protein (JAZ) (PG0004367), auxin influx carrier (AUX) (PG0006550) and auxin response factor (ARF) (PG0005794) all decreased
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