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This prevents transepidermal water loss overnight and keeps the peptide in sustained contact with the skin rather than evaporating amplifying its biological activity during the full repair window

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Immunostaining of GABA A R-1 subunits in the DCN Animals used for brainstem sectioning and subsequent immunohistological evaluations in each experimental group (68 rats/group) were evaluated for sufficient level of sedation after ABR and if necessary given additional ketamine at half the initial dose, then euthanized with i.p