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To identify which of these is required for l-proline transport in our assay conditions, we measured the growth of the corresponding knockout mutants in a growth medium in which we exchanged glycerol with l-proline as described above

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Using the BioGRID database 26 as a reference, we find that interactions of 158 of these targets with human pathogenic viruses have been reported before, which represents a highly significant enrichment among reported interactions between enzymes and viruses (Fishers exact test p = 9.1 10 12 , odds ratio 1.93.4) and supports their central role in viral replication across diverse viruses