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The effect of each treatment on cell viability was assessed by measuring protease activity using the cell permeant substrate GF-AFC using the ApoTox-Glo Triplex Assay

Receptor profile: MT2 acts as a potent agonist at multiple melanocortin receptors, with primary activity at MC1R and MC4R and additional, weaker engagement of MC3R and MC5R, enabling simultaneous study of peripheral pigmentation and central neuroendocrine effects

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Step-by-Step Reconstitution Protocol Temperature equilibration: Allow the lyophilized vial to reach room temperature before opening